attune flow cytometer - An Overview
attune flow cytometer - An Overview
Blog Article
At the moment, the early signals were being dismissed as particles, though the retrospective evaluation demonstrated shared features with the problematic cells in tradition. Tracing the infection assisted the lab establish supplemental laboratory procedures for screening and safety of assay-critical cell traces.
As samples are acquired around the Attune CytPix Flow Cytometer, the higher-speed brightfield digicam captures and suppliers illustrations or photos of detected events at a fee around six,000 visuals for each 2nd, depending on the flow rate and graphic sizing. For higher overall flexibility, Attune Cytometric Software enables you to adjust the graphic seize frequency as desired and select a selected gate for picture capture, if required.
(B) Uncooked 264.seven macrophages are frequently annotated inaccurately with respect to particle rely because of their complexity and noncircular borders. The amount of singlets obtainable for Assessment raises as soon as the consumer trains and employs a far more correct model.
Find new insights into your experimental info by visualizing populations working with combined fluorescence and morphology data. Our program interface wizards help downsampling, concatenation (merged data files) and dimensionality resources which can be created in the software program for an intuitive, streamlined data Evaluation encounter.
*Pricing is indicative only and it is subject matter to change. You should Get in touch with your Agilent representative with any issues. The knowledge set forth herein is not really an offer or simply a commitment, equally of which might be matter to credit score acceptance, conditions and terms, and availability with Agilent. Agilent Money Options is available in picked nations.
Also, it's illuminating to see the quantity of doublets slip via traditional singlet gates, and wonderful to validate the status of unforeseen double-favourable occasions in my Assessment."
Optimizing gating approach. Even sturdy guide singlet gating is mistake-inclined and stays a subjective decision point in Nearly all flow cytometry assays. Imaging may be used to verify and change gates to incorporate only single cells of curiosity.
Reliable graphic good quality even at high throughput Acoustic focusing and also a substantial-speed digicam Merge to picture these T cells continuously at low or superior flow premiums. Effortlessly modify emphasis and camera options to fulfill experimental specifications.
The Attune NxT cytometer may be the reputable workhorse instrument your lab can trust in for dependable and trustworthy general performance. The Attune CytPix product features a higher-velocity brightfield camera and AI-pushed impression Assessment software package, with new upgrades which includes device learning equipment and an intuitive user interface, enabling Improved info precision, precision, and quicker insights into sample biology.
Probably most significantly, these occasions include cells of Evidently distinct phenotypes which could lead on to incorrect conclusions about double positive functions (specifically in exceptional populations).
AI-pushed automatic graphic Examination While using the Attune Cytometric Program interprets occasion attributes into unique morphology parameters which might be coupled with conventional fluorescence and scatter parameters to reveal very reproducible populace modifications and exceptional subsets.
Application note: Automatic picture analysis minimizes consumer-to-consumer variability in flow cytometry gating strategies
Built-in dimensionality reduction tools increased resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to confirm predicted populace resolution in lysed entire human blood. Consumer can see functions from each cluster for Visible affirmation of mobile phenotypes.
Versatile payment plan from Agilent Economical Solutions permit you to get the vital instrumentation you would like for speedy task, or to grow your lab.
Gating on these prolonged parameters helps you to speedily and accurately determine populations of fascination and make sure gating method with little or no handbook overview. The Attune computer software features access to point out-of-the-artwork equipment Finding out Assessment instruments inside an expertly created consumer interface, which makes it extremely convenient to use.
Because the cell line had previously been analyzed to the Attune CytPix Flow Cytometer, the scientists went back to the photographs and have been capable of doc the microbial an infection at the very least 5 times before.
Agilent Benefit Assure assures you at the least 10 years of instrument use from the date you purchase. Otherwise, We're going to credit rating you Using the residual worth of the here method toward an upgraded product.
Listed here, we clearly show that by gating tactics applying picture-based mostly quantitative parameters can distinguish interacting cells from coincident occasions to far more accurately evaluate interacting cells.